Food Safety and Standards Digest

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  *Correspondence Email: priya.sharma@wellness.edu
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Abstract

The increasing global demand for sustainable protein sources has driven extensive research into plant-based alternatives, particularly pulse proteins. This Full Technical Review collates and discusses the recent intellectual updates in advanced extraction and modification techniques for pulse proteins, focusing on methods that enhance solubility and emulsifying capabilities. Specific emphasis is placed on novel applications in meat analogues and dairy-free beverages. We review the techno-functional properties influenced by structural modifications and assess the current state of industrial implementation and regulatory challenges. This comprehensive review aims to provide a critical outlook on future research directions.

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1.0 Introduction

The shifting paradigms in dietary choices necessitate an in-depth understanding of non-traditional protein sources. Pulse proteins, derived from legumes, offer high nutritional value and a lower environmental footprint. This review aims to collate the intellectual knowledge update regarding ...

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2.0 Advanced Extraction Techniques

2.1 Alkaline and Isoelectric Precipitation

2.1.1 Novel Membrane Filtration

Recent studies indicate that the integration of ultrafiltration and diafiltration significantly improves the purity of the protein isolate (Suresh et al., 2014 and so on). This method...

Authors must structure the main body of the manuscript using the following hierarchical heading format:

Level 1 Heading:
Format as 2.0 Advanced Extraction Techniques

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Level 2 Heading:
Format as 2.1 Alkaline and Isoelectric Precipitation

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Level 3 Heading:
Format as 2.1.1 Novel Membrane Filtration

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• A maximum of 8 Tables/Figures/Illustrations are permitted per manuscript (4 Tables and 4 Figures).
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(Sharma, 2002)

(Rakesh & Sharma, 2012)

(Suresh et al., 2014)

(Sharma, 2002; Rakesh & Sharma, 2012; Suresh et al., 2014)

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Formatting Rules:

• Single author: (Sharma, 2002)
• Two authors: (Rakesh & Sharma, 2012)
• Three or more authors: (Suresh et al., 2014)
• Multiple citations in the same parentheses:
  • List in alphabetical order by first author’s surname.
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  • Example: (Rakesh & Sharma, 2012; Sharma, 2002; Suresh et al., 2014)
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3.0 Conclusion

The future trajectory of pulse protein research should focus on...

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References

Gupta, M. (2010). Pharmacological properties and traditional therapeutic uses of important Indian spices: A review. International Journal of Food Properties, 13(5), 1092–1116. https://doi.org/10.1080/10942910902963271

Jiang, T. A. (2019). Health benefits of culinary herbs and spices. Journal of AOAC International, 102(2), 395–411. https://doi.org/10.5740/jaoacint.18-0418

Press Information Bureau. (2025). Trading aromas: The rise of the spice economy. Government of India.

Sharangi, A. B. (Ed.). (2018). Indian spices: The legacy, production and processing of India’s treasured export. Springer.

Srinivasan, K. (2014). Antioxidant potential of spices and their active constituents. Critical Reviews in Food Science and Nutrition, 54(3), 352–372. https://doi.org/10.1080/10408398.2011.585525

• References must be listed in alphabetical order based on the surname of the first author.
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